ProSci

QTRT1 Antibody

Product Code:
 
PSI-59-309
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).
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Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).

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PSI-59-309-400ul400ul£626.00
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Further Information

Additional Names:
Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase, QTRT1, TGT, TGUT
Application Note:
For WB starting dilution is: 1:1000
Background:
tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).
Background References:
  • Chen, Y.C., et al. RNA 16(5):958-968(2010)
  • Deshpande, K.L., et al. Gene 265 (1-2), 205-212 (2001) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This QTRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-371 amino acids from the C-terminal region of human QTRT1.
NCBI Gene ID #:
81890
NCBI Official Name:
Queuine tRNA-ribosyltransferase
NCBI Official Symbol:
QTRT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
Q9BXR0
Protein GI Number:
239938842
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BXR0
User NOte:
Optimal dilutions for each application to be determined by the researcher.