ProSci

GTF2E2 Antibody

Product Code:
 
PSI-57-484
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GTF2E2 using rabbit polyclonal GTF2E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2E2 gene.
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Western blot analysis in A549 cell line lysates (35ug/lane).
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Western blot analysis of GTF2E2 using rabbit polyclonal GTF2E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2E2 gene.
Western blot analysis in A549 cell line lysates (35ug/lane).

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PSI-57-484-400ul400ul£626.00
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Further Information

Additional Names:
Transcription initiation factor IIE subunit beta, TFIIE-beta, General transcription factor IIE subunit 2, GTF2E2, TF2E2
Application Note:
For WB starting dilution is: 1:1000
Background:
GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Background References:
  • Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010)
  • Festen, E.A., et al. Am. J. Gastroenterol. 105(2):395-402(2010)
  • Tanaka, A., et al. Genes Cells 14(3):395-405(2009)
  • Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GTF2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the C-terminal region of human GTF2E2.
NCBI Gene ID #:
2961
NCBI Official Name:
Transcription initiation factor IIE subunit beta
NCBI Official Symbol:
GTF2E2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
P29084
Protein GI Number:
135232
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P29084
User NOte:
Optimal dilutions for each application to be determined by the researcher.