ProSci

NUP85 Antibody

Product Code:
 
PSI-61-273
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-61-273-200ul200ul£626.00
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Further Information

Additional Names:
Nuclear pore complex protein Nup85, 85 kDa nucleoporin, FROUNT, Nucleoporin Nup75, Nucleoporin Nup85, Pericentrin-1, NUP85, NUP75, PCNT1
Application Note:
For WB starting dilution is: 1:2000
Background:
Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade.
Background References:
  • Cronshaw J.M.,et al.J. Cell Biol. 158:915-927(2002).
  • Terashima Y.,et al.Nat. Immunol. 6:827-835(2005).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NUP85 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 163-195 amino acids from human NUP85.
NCBI Gene ID #:
79902
NCBI Official Name:
Nuclear pore complex protein Nup85
NCBI Official Symbol:
NUP85
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
Q9BW27
Protein GI Number:
74733394
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BW27
User NOte:
Optimal dilutions for each application to be determined by the researcher.