ProSci

NTHL1 Antibody

Product Code:
 
PSI-55-909
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in Hela,NCI-H460 cell line lysates (35ug/lane).This demonstrates the NTHL1 antibody detected the NTHL1 protein (arrow).
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Western Blot at 1:1000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela,NCI-H460 cell line lysates (35ug/lane).This demonstrates the NTHL1 antibody detected the NTHL1 protein (arrow).

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PSI-55-909-400ul400ul£626.00
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Further Information

Additional Names:
Endonuclease III-like protein 1 {ECO:0000255|HAMAP-Rule:MF_03183}, hNTH1, 322- {ECO:0000255|HAMAP-Rule:MF_03183}, 429918 {ECO:0000255|HAMAP-Rule:MF_03183}, Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase {ECO:0000255|HAMAP-Rule:MF_03183}, DNA glycoslyase/AP lyase {ECO:0000255|HAMAP-Rule:MF_03183}, NTHL1 {ECO:0000255|HAMAP-Rule:MF_03183}, NTH1, OCTS3
Application Note:
For WB starting dilution is: 1:1000
Background:
The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity.
Background References:
  • Wang, W., et al. Nucleic Acids Res. (2010) In press :
  • Arora, M., et al. Leukemia 24(8):1470-1475(2010)
  • Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
  • Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NTHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human NTHL1.
NCBI Gene ID #:
4913
NCBI Official Name:
Endonuclease III-like protein 1 {ECO:0000255|HAMAP-Rule:MF_03183}
NCBI Official Symbol:
NTHL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
P78549
Protein GI Number:
29840795
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P78549
User NOte:
Optimal dilutions for each application to be determined by the researcher.