ProSci

CPSF7 Antibody

Product Code:
 
PSI-56-247
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-56-247-400ul400ul£626.00
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Further Information

Additional Names:
Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59, CPSF 59 kDa subunit, Pre-mRNA cleavage factor Im 59 kDa subunit, CPSF7
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
CPSF7 is probable a component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
Background References:
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Lim, J., et al. Cell 125(4):801-814(2006)
  • Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004)
  • Zhou, Z., et al. Nature 419(6903):182-185(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This CPSF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 427-455 amino acids from the C-terminal region of human CPSF7.
NCBI Gene ID #:
79869
NCBI Official Name:
Cleavage and polyadenylation specificity factor subunit 7
NCBI Official Symbol:
CPSF7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
Q8N684
Protein GI Number:
74759932
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8N684
User NOte:
Optimal dilutions for each application to be determined by the researcher.