ProSci

APEX1 Antibody

Product Code:
 
PSI-61-551
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-APEX1 Antibody in Hela cell line lysates (35ug/lane)
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Western blot analysis of APEX1 using rabbit polyclonal APEX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.
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Western blot analysis of anti-APEX1 Antibody in Hela cell line lysates (35ug/lane)
Western blot analysis of APEX1 using rabbit polyclonal APEX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.

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PSI-61-551-400ul400ul£626.00
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Further Information

Additional Names:
DNA-(apurinic or apyrimidinic site) lyase, 31--, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial, APEX1, APE, APE1, APEX, APX, HAP1, REF1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This protein is the major AP endonuclease in human cells.
Background References:
  • Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009)
  • Li,W.Q., Carcinogenesis 30 (3), 500-505 (2009)
  • Lo,Y.L., Cancer Epidemiol. Biomarkers Prev. 18 (1), 223-229 (2009)
  • Bhakat,K.K., EMBO J. 22 (23), 6299-6309 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This APEX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human APEX1.
NCBI Gene ID #:
328
NCBI Official Name:
DNA-(apurinic or apyrimidinic site) lyase
NCBI Official Symbol:
APEX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
P27695
Protein GI Number:
113984
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P27695
User NOte:
Optimal dilutions for each application to be determined by the researcher.