ProSci

FARSA Antibody

Product Code:
 
PSI-62-996
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-FARSA Pab in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FARSA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-FARSA Pab in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FARSA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-996-400ul400ul£626.00
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Further Information

Additional Names:
Phenylalanine--tRNA ligase alpha subunit, CML33, Phenylalanyl-tRNA synthetase alpha subunit, PheRS, FARSA, FARS, FARSL, FARSLA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARSA is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This protein has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression.
Background References:
  • Gevaert,K., Nat. Biotechnol. 21 (5), 566-569 (2003)
  • Moor,N.,Protein Expr. Purif. 24 (2), 260-267 (2002)
  • Sen,S., Proc. Natl. Acad. Sci. U.S.A. 94 (12), 6164-6169 (1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This FARSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human FARSA.
NCBI Gene ID #:
2193
NCBI Official Name:
Phenylalanine--tRNA ligase alpha subunit
NCBI Official Symbol:
FARSA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
Q9Y285
Protein GI Number:
12643946
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9Y285
User NOte:
Optimal dilutions for each application to be determined by the researcher.