ProSci

PAAF1 Antibody

Product Code:
 
PSI-61-776
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PAAF1 Antibody in A549 cell line lysates (35ug/lane)
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PAAF1 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of PAAF1 Antibody in A549 cell line lysates (35ug/lane)
PAAF1 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-776-400ul400ul£626.00
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Further Information

Additional Names:
Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71, PAAF1, WDR71
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency.
Background References:
  • Lassot, I., et al. Mol. Cell 25(3):369-383(2007)
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
  • Ponnappan, S., et al. Int. J. Biochem. Cell Biol. 39(4):799-809(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This PAAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the N-terminal region of human PAAF1.
NCBI Gene ID #:
80227
NCBI Official Name:
Proteasomal ATPase-associated factor 1
NCBI Official Symbol:
PAAF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q9BRP4
Protein GI Number:
97217547
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BRP4
User NOte:
Optimal dilutions for each application to be determined by the researcher.