ProSci

MCM4 Antibody

Product Code:
 
PSI-57-795
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis in CEM cell line lysates (35ug/lane).
2 / 2
MCM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in CEM cell line lysates (35ug/lane).
MCM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-795-400ul400ul£626.00
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Further Information

Additional Names:
DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, MCM4, CDC21
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Background References:
  • Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
  • Qian, Z., et al. PLoS Pathog.(3), E1000814 (2010) :
  • Ladstein, R.G., et al. BMC Cancer 10, 140 (2010) :
  • Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Mouse
Immunogen:
This MCM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the C-terminal region of human MCM4.
NCBI Gene ID #:
4173
NCBI Official Name:
DNA replication licensing factor MCM4
NCBI Official Symbol:
MCM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
P33991
Protein GI Number:
68571766
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P33991
User NOte:
Optimal dilutions for each application to be determined by the researcher.