Further Information
Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.
- Rychlik,W., J. Biol. Chem. 262 (22), 10434-10437 (1987)
- Dorfman,J., Genomics(4), 785-788 (1991)
- Pelletier,J., Genomics 10 (4), 1079-1082 (1991)
- Whalen,S.G., J. Biol. Chem. 271 (20), 11831-11837 (1996)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Mouse, Rabbit, Rat, Xenopus
This EIF4E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human EIF4E.
1977
Eukaryotic translation initiation factor 4E
EIF4E
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
P06730
1352435
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
P06730
Optimal dilutions for each application to be determined by the researcher.