ProSci

TCEA1 Antibody

Product Code:
 
PSI-57-969
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis in Hela cell line lysates (35ug/lane).
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TCEA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Hela cell line lysates (35ug/lane).
TCEA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-969-400ul400ul£626.00
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Further Information

Additional Names:
Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIISo, TCEA1, GTF2S, TFIIS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Background References:
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TCEA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the Central region of human TCEA1.
NCBI Gene ID #:
6917
NCBI Official Name:
Transcription elongation factor A protein 1
NCBI Official Symbol:
TCEA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
P23193
Protein GI Number:
1174652
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P23193
User NOte:
Optimal dilutions for each application to be determined by the researcher.