ProSci

ALG14 Antibody

Product Code:
 
PSI-63-693
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of ALG14 Antibody in NCI-H460 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALG14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
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Western blot analysis of ALG14 Antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALG14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-693-400ul400ul£626.00
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Further Information

Additional Names:
UDP-N-acetylglucosamine transferase subunit ALG14 homolog, ALG14
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ALG14 is involved in protein N-glycosylation. It is essential for the second step of the dolichol-linked oligosaccharide pathway. It anchors the catalytic subunit ALG13 to the ER.
Background References:
  • Gao X.-D., et.al., J. Biol. Chem. 280:36254-36262(2005).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ALG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the Central region of human ALG14.
NCBI Gene ID #:
199857
NCBI Official Name:
UDP-N-acetylglucosamine transferase subunit ALG14 homolog
NCBI Official Symbol:
ALG14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
Q96F25
Protein GI Number:
74731649
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96F25
User NOte:
Optimal dilutions for each application to be determined by the researcher.