ProSci

PCAT1 Antibody

Product Code:
 
PSI-63-887
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PCAT1 Antibody in Hela cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PCAT1 Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-887-400ul400ul£626.00
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Further Information

Additional Names:
Lysophosphatidylcholine acyltransferase 1, LPC acyltransferase 1, LPCAT-1, LysoPC acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acetyl-CoA:lyso-PAF acetyltransferase, Lyso-PAF acetyltransferase, LysoPAFAT, Acyltransferase-like 2, Phosphonoformate immuno-associated protein 3, LPCAT1, AYTL2, PFAAP3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PCAT1 acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to hosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis.
Background References:
  • Harayama,T., et.al., J. Lipid Res. 50 (9), 1824-1831 (2009)
  • Mansilla,F., et.al., J. Mol. Med. 87 (1), 85-97 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PCAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human PCAT1.
NCBI Gene ID #:
79888
NCBI Official Name:
Lysophosphatidylcholine acyltransferase 1
NCBI Official Symbol:
LPCAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
Q8NF37
Protein GI Number:
110815902
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8NF37
User NOte:
Optimal dilutions for each application to be determined by the researcher.