ProSci

XPA Antibody

Product Code:
 
PSI-63-642
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of XPA Antibody in NCI-H460 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with XPA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
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Western blot analysis of XPA Antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with XPA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-642-400ul400ul£626.00
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Further Information

Additional Names:
DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein, XPA, XPAC
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
XPA is involved in DNA excision repair. It initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. This protein is required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
Background References:
  • States J.C., et.al., Hum. Mutat. 12:103-113(1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This XPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human XPA.
NCBI Gene ID #:
7507
NCBI Official Name:
DNA repair protein complementing XP-A cells
NCBI Official Symbol:
XPA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
P23025
Protein GI Number:
139816
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P23025
User NOte:
Optimal dilutions for each application to be determined by the researcher.