ProSci

SFRS1 Antibody

Product Code:
 
PSI-62-506
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of SFRS1 Antibody in HL-60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of SFRS1 Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-506-400ul400ul£626.00
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Further Information

Additional Names:
Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit, SRSF1, ASF, SF2, SF2P33, SFRS1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins.
Background References:
  • Sugiyama,N., et.al., Mol. Cell Proteomics(6), 1103-1109 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Chicken, Mouse, Pig
Immunogen:
This SFRS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the C-terminal region of human SFRS1.
NCBI Gene ID #:
6426
NCBI Official Name:
Serine/arginine-rich splicing factor 1
NCBI Official Symbol:
SRSF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
Q07955
Protein GI Number:
730773
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q07955
User NOte:
Optimal dilutions for each application to be determined by the researcher.