ProSci

TSSK4 Antibody

Product Code:
 
PSI-55-734
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of TSSK4 using rabbit polyclonal TSSK4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TSSK4 gene.
2 / 3
TSSK4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of TSSK4 using rabbit polyclonal TSSK4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TSSK4 gene.
TSSK4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-734-400ul400ul£626.00
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Further Information

Additional Names:
Testis-specific serine/threonine-protein kinase 4, TSK-4, TSSK-4, Testis-specific kinase 4, Serine/threonine-protein kinase 22E, TSSK4, C14orf20, STK22E, TSSK5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.
Background References:
  • Aston, K.I., et al. Hum. Reprod. 25(6):1383-1397(2010)
  • Su, D., et al. J. Androl. 29(4):374-378(2008)
  • Zeng, M., et al. BMB Rep 41(4):300-304(2008)
  • Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TSSK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-254 amino acids from the C-terminal region of human TSSK4.
NCBI Gene ID #:
283629
NCBI Official Name:
Testis-specific serine/threonine-protein kinase 4
NCBI Official Symbol:
TSSK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
Q6SA08
Protein GI Number:
62287888
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q6SA08
User NOte:
Optimal dilutions for each application to be determined by the researcher.