ProSci

APEX2 Antibody

Product Code:
 
PSI-63-726
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 4
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 4
Immunofluorescent analysis of U251 cells, using APEX2 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
3 / 4
4 / 4
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Immunofluorescent analysis of U251 cells, using APEX2 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-726-400ul400ul£626.00
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Further Information

Additional Names:
DNA-(apurinic or apyrimidinic site) lyase 2, 31--, AP endonuclease XTH2, APEX nuclease 2, APEX nuclease-like 2, Apurinic-apyrimidinic endonuclease 2, AP endonuclease 2, APEX2, APE2, APEXL2, XTH2
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:25

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
APEX2 may participate in both nuclear and mitochondrial post-replicative base excision repair (BER). In the nucleus functions in the PCNA-dependent BER pathway.
Background References:
  • Hadi,M.Z., et.al., J. Mol. Biol. 316 (3), 853-866 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This APEX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-171 amino acids from the Central region of human APEX2.
NCBI Gene ID #:
27301
NCBI Official Name:
DNA-(apurinic or apyrimidinic site) lyase 2
NCBI Official Symbol:
APEX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q9UBZ4
Protein GI Number:
73921676
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UBZ4
User NOte:
Optimal dilutions for each application to be determined by the researcher.