ProSci

LMO4 Antibody

Product Code:
 
PSI-55-395
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Fluorescent image of A549 cell stained with LMO4 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).LMO4 immunoreactivity is localized to Nucleus significantly.
2 / 3
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
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Fluorescent image of A549 cell stained with LMO4 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).LMO4 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).

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PSI-55-395-400ul400ul£626.00
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Further Information

Additional Names:
LIM domain transcription factor LMO4, Breast tumor autoantigen, LIM domain only protein 4, LMO-4, LMO4
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a cysteine-rich protein that contains two LIM domains but lacks a DNA-binding homeodomain. The encoded protein may play a role as a transcriptional regulator or as an oncogene.
Background References:
  • Tian, Y., et al. Clin. Exp. Metastasis 27(7):455-463(2010)
  • Ribases, M., et al. Biol. Psychiatry 66(10):926-934(2009)
  • Montanez-Wiscovich, M.E., et al. Oncogene 28(41):3608-3618(2009)
  • Huang, Z., et al. Dev. Biol. 327(1):132-142(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This LMO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human LMO4.
NCBI Gene ID #:
8543
NCBI Official Name:
LIM domain transcription factor LMO4
NCBI Official Symbol:
LMO4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
P61968
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
P61968
User NOte:
Optimal dilutions for each application to be determined by the researcher.