ProSci

KIT Antibody

Product Code:
 
PSI-63-108
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of KIT Antibody (Y703) in mouse cerebellum tissue lysates (35ug/lane)
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Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of KIT Antibody (Y703) in mouse cerebellum tissue lysates (35ug/lane)
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-108-400ul400ul£626.00
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Further Information

Additional Names:
Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, KIT, SCFR
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
KIT is the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
Background References:
  • Wardelmann, E., et al., Int. J. Cancer 106(6):887-895 (2003).
  • Lennartsson, J., et al., Exp. Cell Res. 288(1):110-118 (2003).
  • Sakuma, Y., et al., Cancer Sci 94(6):486-491 (2003).
  • Araki, K., et al., Lung Cancer 40(2):173-180 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 680-711 amino acids from human KIT.
NCBI Gene ID #:
3815
NCBI Official Name:
Mast/stem cell growth factor receptor Kit
NCBI Official Symbol:
KIT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
P10721
Protein GI Number:
125472
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Immunology,Neuroscience,Signal Transduction
Swissprot #:
P10721
User NOte:
Optimal dilutions for each application to be determined by the researcher.