ProSci

Mouse Pak4 Antibody

Product Code:
 
PSI-57-745
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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Western blot analysis in Neuro-2a cell line lysates (35ug/lane).
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis in Neuro-2a cell line lysates (35ug/lane).

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PSI-57-745-400ul400ul£626.00
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Further Information

Additional Names:
Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4, Pak4, Kiaa1142
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Pak4 activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2 (By similarity).
Background References:
  • Liu, Y., et al. Oncogene 29(44):5883-5894(2010)
  • Tian, Y., et al. Mech. Dev. 126 (8-9), 710-720 (2009) :
  • Ha, U.H., et al. J. Biol. Chem. 283(45):30624-30631(2008)
  • Liu, Y., et al. Mol. Cancer Res. 6(7):1215-1224(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Pak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the Central region of mouse Pak4.
NCBI Gene ID #:
70584
NCBI Official Name:
Serine/threonine-protein kinase PAK 4
NCBI Official Symbol:
Pak4
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q8BTW9
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
Q8BTW9
User NOte:
Optimal dilutions for each application to be determined by the researcher.