ProSci

Aurora-B Antibody

Product Code:
 
PSI-62-751
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Antibody is used in Western blot to detect STK12 in mouse spleen tissue lysate.
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Western blot analysis of AURKB using rabbit polyclonal Aurora-B (ARK/STK12) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (Lane 2).
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Antibody is used in Western blot to detect STK12 in mouse spleen tissue lysate.
Western blot analysis of AURKB using rabbit polyclonal Aurora-B (ARK/STK12) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (Lane 2).

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PSI-62-751-400ul400ul£626.00
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Further Information

Additional Names:
Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B, AURKB, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. STK12 (Aurora kinase B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes
Background References:
  • Kimura, M., et al., Biochem. Biophys. Res. Commun. 316(3):930-936 (2004).
  • Yasui, Y., et al., J. Biol. Chem. 279(13):12997-13003 (2004).
  • Lampson, M.A., et al., Nat. Cell Biol. 6(3):232-237 (2004).
  • Wheatley, S.P., et al., J. Biol. Chem. 279(7):5655-5660 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Aurora-B (ARK/STK12) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the Central region of human Aurora-B (ARK/STK12).
NCBI Gene ID #:
9212
NCBI Official Name:
Aurora kinase B
NCBI Official Symbol:
AURKB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
Q96GD4
Protein GI Number:
317373473
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
Q96GD4
User NOte:
Optimal dilutions for each application to be determined by the researcher.