ProSci

EHHADH Antibody

Product Code:
 
PSI-63-561
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of lysates from mousr kidney, rat kidney and liver tissue (from left to right), using EHHADH Antibody at 1:1000 at each lane.
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Western blot analysis of EHHADH Antibody in mouse liver(lane 1), kidney(lane 2) tissue lysates (35ug/lane)
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Flow cytometric analysis of HepG2 cells using EHHADH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysates from mousr kidney, rat kidney and liver tissue (from left to right), using EHHADH Antibody at 1:1000 at each lane.
Western blot analysis of EHHADH Antibody in mouse liver(lane 1), kidney(lane 2) tissue lysates (35ug/lane)
Flow cytometric analysis of HepG2 cells using EHHADH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-561-400ul400ul£626.00
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Further Information

Additional Names:
Peroxisomal bifunctional enzyme, PBE, PBFE, Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase, 3-hydroxyacyl-CoA dehydrogenase, EHHADH, ECHD
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity.
Background References:
  • Chen,G.L., et.al., Biochem. Biophys. Res. Commun. 178 (3), 1084-1091 (1991)
  • Lu,Y., et.al., J. Lipid Res. 49 (12), 2582-2589 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This EHHADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.
NCBI Gene ID #:
1962
NCBI Official Name:
Peroxisomal bifunctional enzyme
NCBI Official Symbol:
EHHADH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
79 kDa
Protein Accession #:
Q08426
Protein GI Number:
223590229
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q08426
User NOte:
Optimal dilutions for each application to be determined by the researcher.