ProSci

PKC beta1/2 Antibody

Product Code:
 
PSI-62-591
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Antibody staining PKC beta1/2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: human brain lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of PRKCB using PKC beta1/2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCB gene (Lane 2).
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Antibody staining PKC beta1/2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: human brain lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PRKCB using PKC beta1/2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCB gene (Lane 2).

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PSI-62-591-400ul400ul£626.00
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Further Information

Additional Names:
Protein kinase C beta type, PKC-B, PKC-beta, PRKCB, PKCB, PRKCB1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC beta is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Isoform 1 uses an alternate exon at the 3' end compared to isoform 2, which includes a part of the coding region. The resulting isoform 1 has a distinct and shorter C-terminus, as compared to isoform 2.
Background References:
  • Chen, D., et al., J. Biol. Chem. 279(6):4829-4839 (2004).
  • Hug, B.A., et al., J. Biol. Chem. 279(2):825-830 (2004).
  • Wu, H., et al., Biochem. Biophys. Res. Commun. 311(4):948-953 (2003).
  • Becker, K.P., et al., J. Biol. Chem. 278(52):52747-52754 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PKC beta1/2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-334 amino acids from the Central region of human PKC beta1/2.
NCBI Gene ID #:
5579
NCBI Official Name:
Protein kinase C beta type
NCBI Official Symbol:
PRKCB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
P05771
Protein GI Number:
20141488
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P05771
User NOte:
Optimal dilutions for each application to be determined by the researcher.