ProSci

NDUFA10 Antibody

Product Code:
 
PSI-64-102
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in HepG2 cell line and mouse kidney tissue lysates (35ug/lane).
2 / 3
NDUFA10 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in HepG2 cell line and mouse kidney tissue lysates (35ug/lane).
NDUFA10 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-64-102-400ul400ul£626.00
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Further Information

Additional Names:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, Complex I-42kD, CI-42kD, NADH-ubiquinone oxidoreductase 42 kDa subunit, NDUFA10
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.
Background References:
  • Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
  • Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
  • Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008)
  • Ma, J., et al. Atherosclerosis 191(1):63-72(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NDUFA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-281 amino acids from the Central region of human NDUFA10.
NCBI Gene ID #:
4705
NCBI Official Name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
NCBI Official Symbol:
NDUFA10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
O95299
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
O95299
User NOte:
Optimal dilutions for each application to be determined by the researcher.