ProSci

Mouse Nek8 Antibody

Product Code:
 
PSI-57-357
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in A2058 cell line lysates (35ug/lane).
2 / 3
Western blot analysis of Nek8 using rabbit polyclonal Mouse Nek8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek8 gene.
3 / 3

Western blot analysis in A2058 cell line lysates (35ug/lane).
Western blot analysis of Nek8 using rabbit polyclonal Mouse Nek8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek8 gene.

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PSI-57-357-400ul400ul£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, Nek8, Jck
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans.
Background References:
  • Hellman, N.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18499-18504(2010)
  • Ahmadie, R., et al. J. Nutr. 140(8):1438-1444(2010)
  • Natoli, T.A., et al. Nat. Med. 16(7):788-792(2010)
  • Shiba, D., et al. Cytoskeleton (Hoboken) 67(2):112-119(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Nek8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the C-terminal region of mouse Nek8.
NCBI Gene ID #:
140859
NCBI Official Name:
Serine/threonine-protein kinase Nek8
NCBI Official Symbol:
Nek8
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
Q91ZR4
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q91ZR4
User NOte:
Optimal dilutions for each application to be determined by the researcher.