ProSci

UBE2E2 Antibody

Product Code:
 
PSI-57-295
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse Neuro-2a,Jurkat,Ramos cell line lysates (35ug/lane).
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Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in mouse Neuro-2a,Jurkat,Ramos cell line lysates (35ug/lane).
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

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PSI-57-295-400ul400ul£626.00
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Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 E2, UbcH8, Ubiquitin carrier protein E2, Ubiquitin-protein ligase E2, UBE2E2, UBCH8
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.
Background References:
  • Yamauchi, T., et al. Nat. Genet. 42(10):864-868(2010)
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
  • Ng, D., et al. Int. J. Cancer 123(7):1610-1615(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This UBE2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human UBE2E2.
NCBI Gene ID #:
7325
NCBI Official Name:
Ubiquitin-conjugating enzyme E2 E2
NCBI Official Symbol:
UBE2E2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
Q96LR5
Protein GI Number:
47606201
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q96LR5
User NOte:
Optimal dilutions for each application to be determined by the researcher.