ProSci

HSF1 Sumoylation Site Antibody

Product Code:
 
PSI-61-400
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Jurkat cell line and mouse heart tissue lysates (35ug/lane).
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Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in Jurkat cell line and mouse heart tissue lysates (35ug/lane).
Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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PSI-61-400-400ul400ul£626.00
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Further Information

Additional Names:
Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.
Background References:
  • Hilgarth, et al., Biochem Biophys Res Commun. 2003 Mar 28;303(1):196-200.
  • He, H., et al., J. Biol. Chem. 278(37):35465-35475 (2003).
  • Wang, X., et al., Mol. Cell. Biol. 23(17):6013-6026 (2003).
  • Ignatenko, N.A., et al., Exp. Cell Res. 288(1):1-8 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This HSF1 Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-309 amino acids from human HSF1 Sumoylation Site.
NCBI Gene ID #:
3297
NCBI Official Name:
Heat shock factor protein 1
NCBI Official Symbol:
HSF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q00613
Protein GI Number:
462333
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q00613
User NOte:
Optimal dilutions for each application to be determined by the researcher.