ProSci

ADAMTS18 Antibody

Product Code:
 
PSI-63-696
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of ADAMTS18 Antibody in mouse liver tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ADAMTS18 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ADAMTS18 Antibody in mouse liver tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ADAMTS18 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-696-400ul400ul£626.00
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Further Information

Additional Names:
A disintegrin and metalloproteinase with thrombospondin motifs 18, ADAM-TS 18, ADAM-TS18, ADAMTS-18, 3424-, ADAMTS18, ADAMTS21
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ADAMTS18 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. This protein has a high sequence similarity to the protein encoded by gene ADAMTS16, another family member. It is thought to function as a tumor suppressor.
Background References:
  • Zeng,W., et.al., Biochim. Biophys. Acta 1760 (3), 517-524 (2006)
  • Jin,H., et.al., Oncogene 26 (53), 7490-7498 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ADAMTS18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1066-1095 amino acids from the C-terminal region of human ADAMTS18.
NCBI Gene ID #:
170692
NCBI Official Name:
A disintegrin and metalloproteinase with thrombospondin motifs 18
NCBI Official Symbol:
ADAMTS18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
135 kDa
Protein Accession #:
Q8TE60
Protein GI Number:
296439427
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
Q8TE60
User NOte:
Optimal dilutions for each application to be determined by the researcher.