ProSci

Cellular Apoptosis Susceptibility Antibody

Product Code:
 
PSI-60-497
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 5
Immunohistochemical analysis of paraffin-embedded H. stomach section using CSE1L Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
2 / 5
Fluorescent image of HeLa cells stained with Cellular Apoptosis Susceptibility Antibody . AP1935a was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
3 / 5
4 / 5
Western blot analysis of lysates from Hela, Jurkat, Ramos, mouse NIH/3T3 cell line (from left to right), using CSE1L Antibody at 1:1000 at each lane.
5 / 5
Western blot analysis of anti-CSE1L Pab in, from left to right, A375, CEM, and mouse heart cell line lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H. stomach section using CSE1L Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of HeLa cells stained with Cellular Apoptosis Susceptibility Antibody . AP1935a was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from Hela, Jurkat, Ramos, mouse NIH/3T3 cell line (from left to right), using CSE1L Antibody at 1:1000 at each lane.
Western blot analysis of anti-CSE1L Pab in, from left to right, A375, CEM, and mouse heart cell line lysates (35ug/lane).

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PSI-60-497-400ul400ul£662.00
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Further Information

Additional Names:
Exportin-2, Exp2, Cellular apoptosis susceptibility protein, Chromosome segregation 1-like protein, Importin-alpha re-exporter, CSE1L, CAS, XPO2
Application Note:
For IHC-P starting dilution is: 1:100

For IF starting dilution is: 1:100

For WB starting dilution is: 1:1000
Background:
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.
Background References:
  • Goldberg, G.S., et al., J. Biol. Chem. 278(47):46533-46540 (2003).
  • Behrens, P., et al., Apoptosis 8(1):39-44 (2003).
  • Jiang, M.C., et al., Biochem. Biophys. Res. Commun. 294(4):900-905 (2002).
  • Wellmann, A., et al., Int. J. Mol. Med. 7(5):489-494 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine
Immunogen:
This Cellular Apoptosis Susceptibility antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human Cellular Apoptosis Susceptibility.
NCBI Gene ID #:
1434
NCBI Official Name:
Exportin-2
NCBI Official Symbol:
CSE1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
P55060
Protein GI Number:
62297557
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P55060
User NOte:
Optimal dilutions for each application to be determined by the researcher.