ProSci

SIGLEC12 Antibody

Product Code:
 
PSI-58-424
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of SIGLEC12 Antibody in mouse bladder tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human spleen tissue reacted with SIGLEC12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of SIGLEC12 Antibody in mouse bladder tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human spleen tissue reacted with SIGLEC12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-58-424-400ul400ul£626.00
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Further Information

Additional Names:
Sialic acid-binding Ig-like lectin 12, Siglec-12, Sialic acid-binding Ig-like lectin-like 1, Siglec-L1, SIGLEC12, SIGLECL1, SLG
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. SIGLEC12 is a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. This protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor.
Background References:
  • Angata,T., J. Biol. Chem. 276 (43), 40282-40287 (2001)
  • Yu,Z., J. Biol. Chem. 276 (26), 23816-23824 (2001)
  • Foussias,G., Biochem. Biophys. Res. Commun. 284 (4), 887-899 (2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SIGLEC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 565-595 amino acids from the C-terminal region of human SIGLEC12.
NCBI Gene ID #:
89858
NCBI Official Name:
Sialic acid-binding Ig-like lectin 12
NCBI Official Symbol:
SIGLEC12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q96PQ1
Protein GI Number:
25009264
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology
Swissprot #:
Q96PQ1
User NOte:
Optimal dilutions for each application to be determined by the researcher.