ProSci

HOMER3 Antibody

Product Code:
 
PSI-62-575
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of HOMER3 Antibody in MDA-MB231 cell line lysates (35ug/lane)
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Western blot analysis of HOMER3 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
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Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of HOMER3 using rabbit polyclonal HOMER3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HOMER3 gene.
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Western blot analysis of HOMER3 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Western blot analysis of HOMER3 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of HOMER3 using rabbit polyclonal HOMER3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HOMER3 gene.

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PSI-62-575-400ul400ul£626.00
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Further Information

Additional Names:
Homer protein homolog 3, Homer-3, HOMER3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
HOMER3 is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. This protein binds numerous other proteins including group I metabotropic glutamate receptors,inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.
Background References:
  • Norton,N., et.al., Am. J. Med. Genet.Neuropsychiatr. Genet. 120B (1), 18-21 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HOMER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-308 amino acids from the Central region of human HOMER3.
NCBI Gene ID #:
9454
NCBI Official Name:
Homer protein homolog 3
NCBI Official Symbol:
HOMER3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
Q9NSC5
Protein GI Number:
38605068
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
Q9NSC5
User NOte:
Optimal dilutions for each application to be determined by the researcher.