ProSci

DAG1 Antibody

Product Code:
 
PSI-57-475
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
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DAG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
DAG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-475-400ul400ul£626.00
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Further Information

Additional Names:
Dystroglycan, Dystrophin-associated glycoprotein 1, Alpha-dystroglycan, Alpha-DG, Beta-dystroglycan, Beta-DG, DAG1
Application Note:
For WB starting dilution is: 1:1000-1:2000

For IHC-P starting dilution is: 1:10~50
Background:
Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Nilsson, J., et al. Glycobiology 20(9):1160-1169(2010)
  • Lara-Chacon, B., et al. J. Cell. Biochem. 110(3):706-717(2010)
  • Sgambato, A., et al. Pathology 42(3):248-254(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit, Pig
Immunogen:
This DAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 718-747 amino acids from the C-terminal region of human DAG1.
NCBI Gene ID #:
1605
NCBI Official Name:
Dystroglycan
NCBI Official Symbol:
DAG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q14118
Protein GI Number:
229462879
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q14118
User NOte:
Optimal dilutions for each application to be determined by the researcher.