ProSci

B4GALT5 Antibody

Product Code:
 
PSI-61-901
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the detected B4GALT5 protein (arrow).
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Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the detected B4GALT5 protein (arrow).
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-901-400ul400ul£626.00
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Further Information

Additional Names:
Beta-1,4-galactosyltransferase 5, Beta-1,4-GalTase 5, Beta4Gal-T5, b4Gal-T5, 241-, Beta-1,4-GalT II, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5, B4GALT5
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2. [provided by RefSeq].
Background References:
  • Kitayama, K., et al. J. Biol. Chem. 282(41):30085-30096(2007)
  • Sato, T., et al. J. Biol. Chem. 282(38):27702-27712(2007)
  • Jiang, J., et al. J. Biol. Chem. 281(14):9482-9489(2006)
  • Sato, T., et al. J. Biol. Chem. 279(38):39574-39583(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This B4GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-350 amino acids from the C-terminal region of human B4GALT5.
NCBI Gene ID #:
9334
NCBI Official Name:
Beta-1,4-galactosyltransferase 5
NCBI Official Symbol:
B4GALT5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
O43286
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
O43286
User NOte:
Optimal dilutions for each application to be determined by the researcher.