ProSci

GIT1-S388 Antibody

Product Code:
 
PSI-62-897
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: U-2OS whole cell lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstratesdetected the GIT1 protein (arrow).
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Western Blot at 1:1000 dilution Lane 1: U-2OS whole cell lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstratesdetected the GIT1 protein (arrow).

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PSI-62-897-400ul400ul£626.00
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Further Information

Additional Names:
ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1
Application Note:
For WB starting dilution is: 1:1000
Background:
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (from SwissProt).
Background References:
  • Kawachi, H., et al. Proc. Natl. Acad. Sci. U.S.A. 98(12):6593-6598(2001) Zhao, Z.S., et al. Mol. Cell. Biol. 20(17):6354-6363(2000) Premont, R.T., et al. J. Biol. Chem. 275(29):22373-22380(2000) Premont, R.T., et al. Proc. Natl. Acad. Sci. U.S.A. 95(24):14082-14087(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This GIT1-S388 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the Central region of human GIT1-S388.
NCBI Gene ID #:
28964
NCBI Official Name:
ARF GTPase-activating protein GIT1
NCBI Official Symbol:
GIT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
Q9Y2X7
Protein GI Number:
45645212
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9Y2X7
User NOte:
Optimal dilutions for each application to be determined by the researcher.