ProSci

RSKB Antibody

Product Code:
 
PSI-62-588
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Antibody is used in Western blot to detect MSK2 in placenta tissue lysate. Indirect immunofluorescence analysis showed that MSK2 is predominantly localized in the nucleus of parental (10T1/2) and oncogene-transformed (Ciras-3) mouse fibroblasts. Cells were co-stained with DAPI to visualize nucleus compartment. Data is kindly provided by B. Drobic and Dr. J. Davie from University of Manitoba (Winnipeg, Canada).
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Antibody is used in Western blot to detect MSK2 in placenta tissue lysate. Indirect immunofluorescence analysis showed that MSK2 is predominantly localized in the nucleus of parental (10T1/2) and oncogene-transformed (Ciras-3) mouse fibroblasts. Cells were co-stained with DAPI to visualize nucleus compartment. Data is kindly provided by B. Drobic and Dr. J. Davie from University of Manitoba (Winnipeg, Canada).

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PSI-62-588-400ul400ul£626.00
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Further Information

Additional Names:
Ribosomal protein S6 kinase alpha-4, S6K-alpha-4, 90 kDa ribosomal protein S6 kinase 4, Nuclear mitogen- and stress-activated protein kinase 2, Ribosomal protein kinase B, RSKB, RPS6KA4, MSK2
Application Note:
For WB starting dilution is: 1:1000
Background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).
Background References:
  • Zhu, S., et al., Hum. Genet. 103(6):674-680 (1998).
  • Pierrat, B., et al., J. Biol. Chem. 273(45):29661-29671 (1998).
  • Deak, M., et al., EMBO J. 17(15):4426-4441 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RSKB (MSK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-354 amino acids from the C-terminal region of human RSKB (MSK2).
NCBI Gene ID #:
8986
NCBI Official Name:
Ribosomal protein S6 kinase alpha-4
NCBI Official Symbol:
RPS6KA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
86 kDa
Protein Accession #:
O75676
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
O75676
User NOte:
Optimal dilutions for each application to be determined by the researcher.