Further Information
Myosin light chain kinase, smooth muscle, MLCK, smMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
- Lazar, V., et al., Genomics 57(2):256-267 (1999). Watterson, D.M., et al., J. Cell. Biochem. 75(3):481-491 (1999). Garcia, J.G., et al., Am. J. Respir. Cell Mol. Biol. 16(5):489-494 (1997). Potier, M.C., et al., Genomics 29(3):562-570 (1995).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Rb
This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 908-938 amino acids from the N-terminal region of human MLCK.
4638
Myosin light chain kinase, smooth muscle
MYLK
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
211 kDa
Q15746
300669714
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Signal Transduction
Q15746
Optimal dilutions for each application to be determined by the researcher.