ProSci

PHB2 Antibody

Product Code:
 
PSI-62-781
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of PHB2-Y128in mouse NIH-3T3 cell line lysates (35ug/lane)
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PHB2-Y128in mouse NIH-3T3 cell line lysates (35ug/lane)

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PSI-62-781-400ul400ul£626.00
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Further Information

Additional Names:
Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, PHB2 {ECO:0000312|EMBL:AAH147661, ECO:0000312|HGNC:HGNC:30306}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.
Background References:
  • Takata,H., Curr. Biol. 17 (15), 1356-1361 (2007)
  • Kasashima,K., J. Biol. Chem. 281 (47), 36401-36410 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rat
Immunogen:
This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from human PHB2.
NCBI Gene ID #:
11331
NCBI Official Name:
Prohibitin-2
NCBI Official Symbol:
PHB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
Q99623
Protein GI Number:
74752151
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q99623
User NOte:
Optimal dilutions for each application to be determined by the researcher.