ProSci

RSK2 Antibody

Product Code:
 
PSI-63-300
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis in Ramos cell line and mouse thymus tissue lysates (35ug/lane).
2 / 2
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with RSK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Western blot analysis in Ramos cell line and mouse thymus tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with RSK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-300-400ul400ul£626.00
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Further Information

Additional Names:
Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, 90 kDa ribosomal protein S6 kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated protein kinase 1, ISPK-1, MAP kinase-activated protein kinase 1b, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, Ribosomal S6 kinase 2, RSK-2, pp90RSK2, RPS6KA3, ISPK1, MAPKAPK1B, RSK2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
RSK3 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in the gene have been associated with Coffin-Lowry syndrome (CLS).
Background References:
  • Yang, X., et al., Cell 117(3):387-398 (2004).
  • Guimiot, F., et al., Gene Expr. Patterns 4(1):111-114 (2004).
  • Zeniou, M., et al., (er) Nucleic Acids Res. 32(3):1214-1223 (2004).
  • Vaidyanathan, H., et al., J. Biol. Chem. 278(34):32367-32372 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RSK2 (RPS6KA3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human RSK2 (RPS6KA3).
NCBI Gene ID #:
6197
NCBI Official Name:
Ribosomal protein S6 kinase alpha-3
NCBI Official Symbol:
RPS6KA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
P51812
Protein GI Number:
1730070
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P51812
User NOte:
Optimal dilutions for each application to be determined by the researcher.