ProSci

NCOA7 Antibody

Product Code:
 
PSI-63-996
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human brain lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).
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Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-996-400ul400ul£626.00
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Further Information

Additional Names:
Nuclear receptor coactivator 7, 140 kDa estrogen receptor-associated protein, Estrogen nuclear receptor coactivator 1, NCOA7, ERAP140, ESNA1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
NCOA7 enhances the transcriptional activities of several nuclear receptors. It is involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
Background References:
  • Arai, H., et al. Oncol. Rep. 19(6):1381-1388(2008)
  • Liu, Y., et al. Schizophr. Res. 89 (1-3), 360-361 (2007) :
  • Durand, M., et al. BMC Cell Biol. 8, 13 (2007) :
  • Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7.
NCBI Gene ID #:
135112
NCBI Official Name:
Nuclear receptor coactivator 7
NCBI Official Symbol:
NCOA7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
106 kDa
Protein Accession #:
Q8NI08
Protein GI Number:
110287684
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q8NI08
User NOte:
Optimal dilutions for each application to be determined by the researcher.