ProSci

MAN2A2 Antibody

Product Code:
 
PSI-56-467
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
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MAN2A2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
MAN2A2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-467-400ul400ul£626.00
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Further Information

Additional Names:
Alpha-mannosidase 2x, Alpha-mannosidase IIx, Man IIx, Mannosidase alpha class 2A member 2, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, MAN2A2, MANA2X
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
MAN2A2 catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Background References:
  • Lesch, K.P., et al.Neural Transm 115(11):1573-1585(2008)
  • Stelzl, U., et al. Cell 122(6):957-968(2005)
  • Hillman, R.T., et al. Genome Biol.(2), R8 (2004) :
  • Misago, M., et al. Proc. Natl. Acad. Sci. U.S.A. 92(25):11766-11770(1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MAN2A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 767-796 amino acids from the Central region of human MAN2A2.
NCBI Gene ID #:
4122
NCBI Official Name:
Alpha-mannosidase 2x
NCBI Official Symbol:
MAN2A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
131 kDa
Protein Accession #:
P49641
Protein GI Number:
347595795
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P49641
User NOte:
Optimal dilutions for each application to be determined by the researcher.