ProSci

RRAGD Antibody

Product Code:
 
PSI-61-732
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 5
Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 5
Western blot analysis of RRAGD Antibody in MDA-MB435 cell line lysates (35ug/lane)
3 / 5
4 / 5
RRAGD Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
5 / 5
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of RRAGD Antibody in MDA-MB435 cell line lysates (35ug/lane)
RRAGD Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-732-400ul400ul£626.00
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Further Information

Additional Names:
Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903)
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.
Background References:
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • de Wit, N.J., et al. Br. J. Cancer 92(12):2249-2261(2005)
  • Sekiguchi, T., et al. J. Biol. Chem. 279(9):8343-8350(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD.
NCBI Gene ID #:
58528
NCBI Official Name:
Ras-related GTP-binding protein D
NCBI Official Symbol:
RRAGD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q9NQL2
Protein GI Number:
74752904
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9NQL2
User NOte:
Optimal dilutions for each application to be determined by the researcher.