ProSci

GTF2A1 Antibody

Product Code:
 
PSI-59-357
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
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Western blot analysis in mouse kidney tissue lysates (35ug/lane).

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PSI-59-357-400ul400ul£626.00
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Further Information

Additional Names:
Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42, Transcription initiation factor IIA alpha chain, TFIIA p35 subunit, Transcription initiation factor IIA beta chain, TFIIA p19 subunit, GTF2A1, TF2A1
Application Note:
For WB starting dilution is: 1:1000
Background:
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.
Background References:
  • Huang, Y.W., et al. Oncol. Rep. 22(4):853-861(2009)
  • Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
  • Hieb, A.R., et al. J. Mol. Biol. 372(3):619-632(2007)
  • Lee, K.A., et al. Cytokine 38(2):96-100(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This GTF2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the C-terminal region of human GTF2A1.
NCBI Gene ID #:
2957
NCBI Official Name:
Transcription initiation factor IIA subunit 1
NCBI Official Symbol:
GTF2A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P52655
Protein GI Number:
1711663
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P52655
User NOte:
Optimal dilutions for each application to be determined by the researcher.