ProSci

CTLA 4 Recombinant Protein

Product Code:
 
PSI-RF16010-01
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
 

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PSI-RF16010-01-0.1mg0.1mg£433.00
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Further Information

Additional Names:
CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Background References:
  • Waterhouse P, et al., 1995, Science 270 (5238): 985?8.
  • Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596?602.
  • Rudd, CE. et al., 2009, Immunol. Rev.?229?(1): 12-26.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein at 2μg/mL (100μL/well) can bind rhB7-1/ CD80 Protein Fc Chimera with a linear range of 2 - 50 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein at 10μg/mL (100μL/well) can bind rh PD-L1 /B7-H1 /CD274 Protein Fc Chimera with a linear range of 20 - 10000 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
1493
NCBI Official Name:
cytotoxic T-lymphocyte-associated protein 4
NCBI Official Symbol:
CTLA4
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
14.3 kDa
Protein Accession #:
NP_005205.2
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P16410

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