ProSci

PDL2 Antibody [8C12]

Product Code:
 
PSI-RF16022
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
8C12
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
PD-L2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml
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Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.
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Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL. <br><br>Green: PDL2 Antibody [8C12] (RF16022) <br> Blue: DAPI staining
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Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL. <br><br>Green: PDL2 Antibody [8C12] (RF16022) <br> Blue: DAPI staining
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Immunofluorescence of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 20 μg/mL. <br><br>Green: PDL2 Antibody [8C12] (RF16022) <br> Blue: DAPI staining
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Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.
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Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.
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Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.
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Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml
Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.
Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL. <br><br>Green: PDL2 Antibody [8C12] (RF16022) <br> Blue: DAPI staining
Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL. <br><br>Green: PDL2 Antibody [8C12] (RF16022) <br> Blue: DAPI staining
Immunofluorescence of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 20 μg/mL. <br><br>Green: PDL2 Antibody [8C12] (RF16022) <br> Blue: DAPI staining
Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.
Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.
Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.

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CodeSizePrice
PSI-RF16022-0.02mg0.02mg£150.00
Quantity:
PSI-RF16022-0.1mg0.1mg£515.00
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Further Information

Additional Names:
PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2
Application Note:
PD-L2 antibody can be used for detection of PD-L2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Background:
PD-L2 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.
Background References:
  • Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
  • Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
  • LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
  • Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.
Buffer:
PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PD-L2 antibody was raised against the extracellular domain of human PD-L2.
NCBI Gene ID #:
80380
NCBI Official Name:
programmed cell death 1 ligand
NCBI Official Symbol:
PDCD1LG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 30 kDa

Observed: 38 kDa
Protein Accession #:
NP_079515
Protein GI Number:
190014605
Purification:
PD-L2 Antibody is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
P9BQ51
User NOte:
Optimal dilutions for each application to be determined by the researcher.