Product Code:
CO20451-0.1
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunoblotting
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Radioimmunoassay (RIA)
- Western Blot (WB)
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This product comes from:
Netherlands.
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7-10 working days.
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Further Information
Applications Description:
IHC(P), IFA, ELISA, RIA, IB/WB
Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type IV 100%; murine collagen type I, II and III <0.1%; human collagen type IV and V <0.1%; murine fibronectin <0.1%; murine laminin <0.1% in RIA at 1:5000 dilution.
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
app. 1 mg/ml
Purified collagen type IV from murine tumour tissue
IHC(P) 60 min at RT or 2-8°C over night
Mouse skin or liver
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Mouse
P02463 (CO4A1_MOUSE)
(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
