NSJ Bioreagents

MYBL2 Antibody

Product Code:
 
NSJ-R31028
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MYBL2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE mouse liver tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat liver tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human tonsil tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
Western blot testing of human Raji cell lysate with MYBL2 antibody. Predicted molecular weight: ~79 kDa, may also be observed at 100~110 kDa.
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IHC staining of FFPE mouse liver tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate with MYBL2 antibody. Predicted molecular weight: ~79 kDa, may also be observed at 100~110 kDa.

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NSJ-R31028-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MYBL2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
V-MYB avian myeloblastosis viral oncogene homolog-like 2, also called MYB-related gene B-MYB, is a protein that in humans is encoded by the MYBL2 gene. The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. Barletta et al.(1991) assigned the gene to chromosome Xq13. However, Noben-Trauth et al.(1996) demonstrated that this assignment was an error. Using mouse Mybl2 cDNA clones as probes, they assigned the gene in an interspecific backcross panel to distal mouse chromosome 2. Using human cDNA probes in combination with fluorescence in situ hybridization analysis, they localized MYBL2 to chromosome 20q13.1, a region that is commonly deleted in myeloid disorders and shows high homology of Synteny to mouse chromosome 2. It has been shown to activate the cell division cycle 2, Cyclin D1, and Insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human MYBL2/B-MYB (MSRRTRCEDLDELHYQD) was used as the immunogen for this MYBL2 antibody (100% mouse homology).
Limitation:
This MYBL2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P10244