NSJ Bioreagents

N-Cadherin Antibody

Product Code:
 
NSJ-R30391
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the N-Cadherin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 8
Immunofluorescent staining of FFPE human colon cancer with N-Cadherin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
2 / 8
Immunofluorescent staining of FFPE rat heart tissue with N-Cadherin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
3 / 8
IHC staining of FFPE human tonsil tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human ovarian cancer tissue with N-Cadherin antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE mouse heart tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE rat heart tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of 1) rat brain, 2) rat heart, 3) rat liver, 4) mouse heart, 5) mouse liver and 6) human SK-O-V3 cell lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).
8 / 8
Flow cytometry testing of human HeLa cells with N-Cadherin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= N-Cadherin antibody.
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Immunofluorescent staining of FFPE human colon cancer with N-Cadherin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE rat heart tissue with N-Cadherin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
IHC staining of FFPE human tonsil tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with N-Cadherin antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain, 2) rat heart, 3) rat liver, 4) mouse heart, 5) mouse liver and 6) human SK-O-V3 cell lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).
Flow cytometry testing of human HeLa cells with N-Cadherin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= N-Cadherin antibody.

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CodeSizePrice
NSJ-R30391-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the N-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NCAD is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). Human and mouse nucleotide sequences are 96% identical. The mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. The human Ncad gene contains 16 exons and its sequence is highly similar to both the mouse gene (including the large first and second introns) and other cadherin genes. Cadherins regulates dendritic spine morphogenesis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 701-714 (CQCDSNGDCTDVDR) were used as the immunogen for this N-Cadherin antibody (100% homologous human, mouse and rat).
Limitation:
This N-Cadherin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P19022