NSJ Bioreagents

NKCC2 Antibody (SLC12A1)

Product Code:
 
NSJ-R31289
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NKCC2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of NKCC2 antibody and rat kidney tissue lysate. Expected/observed size ~121KD
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IHC-P: NKCC2 antibody testing of rat kidney tissue
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IHC-F testing of NKCC2 antibody and rat kidney tissue
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Western blot testing of NKCC2 antibody and rat kidney tissue lysate. Expected/observed size ~121KD
IHC-P: NKCC2 antibody testing of rat kidney tissue
IHC-F testing of NKCC2 antibody and rat kidney tissue

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CodeSizePrice
NSJ-R31289-100ug100 ug£535.00
Quantity:
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the NKCC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Solute carrier family 12 (sodium/potassium/chloride transporters), member 1, also called NKCC2 is specifically found in cells of the thick ascending limb of the loop of Henle in nephrons, the basic functional units of the kidney. This gene is mapped to 15q21.1. This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. NKCC2 plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. This gene plays a vital role in the regulation of ionic balance and cell volume.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human SLC12A1/NKCC2 (KVNRPSLLEIHEQLA) was used as the immunogen for this NKCC2 antibody (100% mouse homology).
Limitation:
This NKCC2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13621