NSJ Bioreagents

PAH Antibody / Phenylalanine hydroxylase

Product Code:
 
NSJ-R32863
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the PAH antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human liver cancer tissue with PAH antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE mouse kidney tissue with PAH antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat kidney tissue with PAH antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human HepG2, 2) human Caco-2, 3) rat kidney, 4) rat liver, 5) mouse kidney and 6) mouse liver tissue lysate with PAH antibody. Predicted molecular weight ~52 kDa.
5 / 5
Flow cytometry testing of human HepG2 cells with PAH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAH antibody.
❮❯

IHC staining of FFPE human liver cancer tissue with PAH antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with PAH antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with PAH antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HepG2, 2) human Caco-2, 3) rat kidney, 4) rat liver, 5) mouse kidney and 6) mouse liver tissue lysate with PAH antibody. Predicted molecular weight ~52 kDa.
Flow cytometry testing of human HepG2 cells with PAH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAH antibody.

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CodeSizePrice
NSJ-R32863-100ug100 ug£535.00
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Further Information

Application Details:
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PAH antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Phenylalanine hydroxylase (PAH) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids R71-H208 was used as the immunogen for the PAH antibody.
Limitation:
This PAH antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00439