NSJ Bioreagents

PDK2 Antibody

Product Code:
 
NSJ-R31101
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PDK2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
Western blot testing of PDK2 antbody on Lane 1: rat heart; 2: HeLa; 3: SW620; 4: MCF-7 cell lysate. Expected/observed size ~46KD
2 / 4
IHC-P: PDK2 antibody testing of human breast cancer tissue
3 / 4
IHC-P: PDK2 antibody testing of rat brain tissue
4 / 4
ICC testing of PDK2 antbody and HeLa cells
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Western blot testing of PDK2 antbody on Lane 1: rat heart; 2: HeLa; 3: SW620; 4: MCF-7 cell lysate. Expected/observed size ~46KD
IHC-P: PDK2 antibody testing of human breast cancer tissue
IHC-P: PDK2 antibody testing of rat brain tissue
ICC testing of PDK2 antbody and HeLa cells

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CodeSizePrice
NSJ-R31101-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Pyruvate dehydrogenase kinase isoenzyme 2, is an enzyme that in humans is encoded by the PDK2 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. The human PDK2 gene encodes a protein with a predicted molecular mass of 46,181 Da that shares 96% identity with that of the rat gene. Northern blot analysis showed that the PDK2 message was found in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human PDK2 (WRHYQTIQEAGDWC) was used as the immunogen for this PDK2 antibody (100% homologous in human, mouse and rat).
Limitation:
This PDK2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15119